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H9661
Sigma
Hepatocyte Growth Factor human
recombinant, expressed in mouse NSO cells, lyophilized powder, cell culture tested
| Synonym: | Hepatopoietin A, HGF, Scatter Factor |
| MDL number: | MFCD00217422 |
Description
| Analysis Note | The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in the HGF responsive monkey epithelial cell line, 4MBr-5. |
| Biochem/physiol Actions | Hepatocyte growth factor is a potent mitogen for hepatocytes and a variety of cell types including endothelial and epithelial cells, melanocytes, and keratinocytes. It also mediates epithelial morphogenesis. HGF is the ligand for the tyrosine kinase receptor encoded by the met proto-oncogene. |
| Other Notes | The NSO-expressed recombinant HGF is greater than 95% heterodimeric HGF. The insect cell line-expressed recombinant HGF is a mixture of single chain HGF (predominant) with some heterodimeric HGF. Single chain HGF and heterodimeric HGF are equally active in in vitro assays. |
| Physical form | Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing 50 μg of bovine serum albumin per 1μg of cytokine. |
Properties
| recombinant | expressed in mouse NSO cells |
| assay | ≥95% (SDS-PAGE) |
| form | lyophilized powder |
| mol wt | mol wt 70-80 kDa by SDS-PAGE (non-reducing) |
| total impurities | Endotoxin, tested |
| suitability | cell culture tested |
| Gene Information | human ... HGF(3082) |
| storage temp. | −20°C |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, type N95 (US), type P1 (EN143) respirator filter |
| WGK Germany | 3 |
